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Immortalized cell lines

Public Group July 29, 2025

cailynn johnson Immortalized cell lines are genetically modified cells capable of indefinite proliferation, making them ideal for long-term and high-throughput experiments. Unlike primary cells, which closely mimic in vivo conditions but have limited lifespan, immortalized lines offer consistency, scalability, and ease of cultivation.

iPSC-derived blood-brain barrier

Public Group July 29, 2025

cailynn johnson iPSC-derived blood-brain barrier (BBB) models replicate human BBB structure using stem cell-derived endothelial cells, astrocytes, and pericytes. These models offer high human specificity and customizable features for drug screening, disease modeling, and permeability prediction.

Brain spheroids

Public Group July 29, 2025

cailynn johnson Brain spheroids are 3D cellular models derived from human stem cells that mimic the structure and function of the brain. They include neurons, astrocytes, and endothelial cells, forming a microenvironment that replicates blood-brain barrier (BBB) properties.

Neural differentiation

Public Group July 29, 2025

cailynn johnson Neural differentiation involves guiding pluripotent stem cells to become specific neural cell types such as neurons, astrocytes, and oligodendrocytes. This process uses signaling molecules like retinoic acid and BMP to activate neural genes and form neural rosettes.

Clier-qPCR

Public Group July 29, 2025

cailynn johnson Clier-qPCR is a sensitive method for validating and quantifying glycosylated RNAs using click chemistry and real-time PCR. It enriches glycoRNAs through biotin labeling and magnetic bead capture, followed by ΔΔCt-based quantification.

GlycoRNA imaging for glycorna analysis

Public Group July 29, 2025

cailynn johnson GlycoRNA imaging enables spatial visualization of glycosylated RNAs at the single-cell level. Using dual-recognition probes and signal amplification, this technique detects glycoRNAs on cell surfaces and within subcellular structures.

GlycoRNA-seq

Public Group July 29, 2025

cailynn johnson GlycoRNA-seq is a specialized sequencing method designed to detect and analyze glycosylated small noncoding RNAs. Unlike traditional RNA-seq, it enriches for RNAs modified with glycans—especially those containing sialic acid and fucose—using techniques like metabolic labeling and click chemistry.

GlycoRNA analysis

Public Group July 29, 2025

cailynn johnson GlycoRNA analysis explores glycosylated RNAs—small noncoding molecules modified with N-linked glycans that influence immune signaling and cellular communication. Advanced techniques like GlycoRNA-seq, imaging, and Clier-qPCR enable researchers to identify, visualize, and quantify these RNAs across tissues and cell types.

unblockedgames

Public Group July 21, 2025

Casey_Flowers

Are you looking for a fun and easy way to take a break during school or work hours? If so, unblocked games might be exactly what you need. These are online games that can be played directly in your browser, even on networks that usually restrict gaming websites. Whether you're a student trying to unwind between classes or an employee needing a quick mental refresh, unblocked games offer a convenient and entertaining option without the need for downloads or special software.

ChatGPT OpenAI

Public Group July 20, 2025

chatgptopenai2

Die digitale Landschaft verändert sich rasant, und künstliche Intelligenz spielt dabei eine immer zentralere Rolle. Doch oft scheinen diese fortschrittlichen Technologien mit hohen Kosten oder einer komplexen Einarbeitung verbunden zu sein. Hier setzt ein entscheidender Trend an: der freie Zugang zu leistungsstarken KI-Tools.

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